Single Cell Analysis & CyTOF

 

Single Cell Genomic Analysis. Single cell genomic studies can be performed using the Fluidigm C1 instrument within the Parnassus Flow Core.  The C1 is a single cell isolation device that uses an integrated fluidic circuit (IFC) chip to capture each cell's individual genomic content for downstream genomic analysis.     

The C1 allows you to prepare single cell templates for mRNA sequencing, DNA sequencing, epigenetics or miRNA expression. This single cell isolator utilizes an optically clear IFC chip which allows you to stain captured cells and examine them by microscopy for viability, surface markers, or reporter genes, as well as confirm there is 1 cell in each capture site.  Upon confirmation of singlet capture efficiency, the C1 lyses the cell and amplifies the targeted genomic content based on chip selection and reagents added, which you will then isolate for your genomic analysis.  The C1 is operated using C1 touchscreen software and is available for all labs to use.  For more information about single cell analysis please visit the Parnassus Flow Cytometry CoreIf you wish to use the Fluidigm C1 and receive a Liver Center discount, please contact the Liver Center administration here before scheduling work. 

Fluidigm C1 - Parnassus Flow Core

 

 

CyTOF. Cell analysis by mass spectrometry can be peformed using the the Fluidigm CyTOF instrument within the Parnassus Flow Core.  CyTOF is a mass spectrometry cytometer analyzer capable of generating data of 46 parameters for each event passing through the detector.

The workflow and operation are very similar to flow cytometry, but rather than using fluorescence, elemental tags are utilized.  Analysis occurs in the Time-of-Flight (TOF) chamber, allowing detection of over 40 different parameters simultaneously. It is capable of analysis rates of up to 500 events per second.  This instrument is useful for discovery experiments because it can capture large amounts of data with limited amounts of input sample.  It is operated using CyTOF Acquisition Software and is available for all labs to use. For more information about CyTOF please visit the Parnassus Flow Cytometry CoreIf you wish to use the CyTOF instrument and receive a Liver Center discount, please contact the Liver Center administration here before scheduling work. 

CyTOF - Parnassus Flow Core