Quantstudio 6 Flex Real-Time PCR. The Immunology Core is equpped with an in-house Quantstudio 6 Flex Real-Time PCR System on the Parnassus campus (S-357). This instrument accommodates both 96-well and 384-well plates and detects 5 colors for use with SYBR and Taqman assays. The 384-well format uses smaller amounts of reagents and cDNA than the 96-well format, which increases output per sample and saves money on reagents. This feature is very important for those who need to analyze the small amount of RNA derived from liver biopsy tissue.
Liver Center members have priority acess to this instrument. Users are assessed an annual fee, prorated to the number of usage hours. To schedule use of the instrument click here. For information about pricing, inquire here.
Fluidigm Biomark HD. High-throughput QPCR can be performed using the Fluidigm Biomark HD system within the Parnassus Flow Core. The Biomark HD uses integrated fludic circuits (IFCs) to enable genotyping, gene expression profiling and quantitative real-time digital PCR, all on a nanoliter scale. Usage rates for this instrument are are discounted for Liver Center members. To learn more about the Biomark HD, click here. If you wish to use the Fluidigm Biomark HD and receive a Liver Center discount, please contact the Liver Center administration here before scheduling work. To schedule work on this instrument, please contact the Parnassus Flow Cytometry Core here.