Keith Mostov, MD, PhD
Research Interests
The Mostov laboratory studies epithelial morphogenesis and polarity in several organs including the liver. One of our interests is the mechanism by which cholangiocytes differentiate from hepatoblasts and progress to form ductal plates and eventually branching tubular networks. We recently developed a culture system in which a liver progenitor cell line, HPPL, reorganizes from a monolayer to tubular structures by being overlaid with a gel containing type I collagen and Matrigel. HPPL assumed a double layer configuration in the specialized culture environment and then developed a luminal space. Formation of the double layer does not involve cholangiocyte proliferation, but is associated with phosphatidylinositol 3-kinase and Akt activity, as well as transcriptional activity of HNF1.
Selected Publications
- Gassama A Yu W, Martin F, Kierbel A, Engel J, Mostov K. Phosphatidylinositol 3,4,5-trisphosphate regulates the formation of the basolateral plasma membrane in epithelial cells. Nat Cell Biol 2006;8:963-970.
- Tanimizu N, Miyajima A, Mostov KE.Liver progenitor cells develop cholangiocyte-type epithelial polarity in three-dimensional culture. Mol Biol Cell. 2007;18:1472-1479.
- Bagnat M, Cheung ID, Mostov KE, Stainier DY. Genetic control of single lumen formation in the zebrafish gut. Nat Cell Biol 2007;9:954-960.
- Martin-Belmonte F, Gassama A, Datta A, Yu W, Gerke V, Mostov, K. PTEN-mediated apical segregation of phosphoinositides controls epithelial polarity through Cdc42. Cell 2007;128:383-397.
- Professor
- Anatomy, and Biochemistry and Biophysics
Research Theme
- Hepatic Physiology and Metabolism
Contact Information
- Keith.mostov@
ucsf.edu - Phone: (415) 476-6048
- Fax: (415) 514-0169
- Genentech Hall
- 600 16th St.
- Box 214, N212B
- San Francisco, CA. 94158-2517
Other UCSF Affiliations
- PIB.S. graduate program
- BMS graduate program
- California Institute for Regeneration Medicine
- Cardiovascular Research Institute
- Hellen Diller Family Comprehensive Cancer Center