The Cell Biology Core prepares primary hepatocytes from rats and mice using in situ collagenase perfusion. Cell suspensions are purified by several methods, and clients have the option of choosing the method that suits their experimental needs (e.g. Percoll gradient, centrifugal elutriation, FACS). Clients are encouraged to consult with Core personnel for optimal service.
Hepatocytes are provided as cell suspensions or as freshly seeded cultures on collagen-coated plates in serum-containing medium. Custom culture conditions can be provided upon request.
Prices for cell isolation, cell purification and culture can be found here.